Computational protocol: Predicted Coverage and Immuno-Safety of a Recombinant C-Repeat Region Based Streptococcus pyogenes Vaccine Candidate

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Protocol publication

[…] The secondary structure of 14mer peptides in solution was investigated by circular dichromic spectra acquired with a JASCO J-715 CD Spectropolarimeter at 20°C. Peptide solutions were assessed at 0.02 mg/mL in 10 mM sodium phosphate, 20 mM NaCl, pH 8.0. Far UV-CD spectra were acquired at from 260 to 190 nm in 0–50% 2,2-trifluoroethanol (TFE). All spectra were corrected for buffer baseline and converted to mean residue ellipticity (θ) using the software ACDP v2.9 [] and compared with the software SDAR v2.9 []. The amount of TFE required to observe alpha-helical secondary structure is based on the appearance of a peak at 208 nm. For each peptide, secondary structure was predicted based on its amino acid sequence using the software PSIPRED []. […]

Pipeline specifications

Software tools ACDP, SDAR, PSIPRED
Applications Miscellaneous, Circular dichroism spectroscopy
Organisms Mus musculus, Rattus norvegicus, Streptococcus pyogenes, Cell fusing agent virus
Diseases Rheumatic Heart Disease