|Application:||Gene expression microarray analysis|
|Number of samples:||8|
|Release date:||Mar 21 2012|
|Last update date:||Dec 6 2012|
|Chemicals:||Naphthoquinones, Vitamin K 3|
|Dataset link||Extension of lifespan in C. elegans by naphthoquinones that act through stress hormesis mechanisms|
Approximately 1,000 age-synchronized young adults were treated for two days with either 100 µM plumbagin or DMSO as a vehicle control and then washed three times in M9 and flash frozen with dry ice. RNA was isolated with the Absolutely RNA Miniprep Kit (Agilent Technologies, USA). RNA concentration and quality was assessed using an Agilent Bioanalyzer 2100 (Agilent Technologies, USA). Microarray hybridizations were performed with the C. elegans 4x44K Oligo Microarray (Agilent Technologies, USA). Raw microarray hybridization intensity data from four separate experiments were log-transformed and normalized to generate z-scores and subsequent z-ratios (Cheadle et al. 2003).
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