|Application:||Gene expression microarray analysis|
|Number of samples:||6|
|Release date:||Apr 24 2008|
|Last update date:||Aug 28 2018|
|Dataset link||Expression analysis of the effect of protoplasting and FACS sorting in roots exposed to iron deficiency (-Fe)|
To estimate the effect that protoplasting and cell sorting has on the expression of -Fe regulated genes we prepared samples as in Birnbaum et al. (2005) Nat. Methods, except that all cells were collected after cell sorting. Cells were collected from roots that had been exposed to iron deficient (-Fe) conditions (0.3mM Ferrozine in MS media containing no ferrous sulfate) for 24 hours prior to protoplasting. Whole roots were also collected after a similar treatment regimen with -Fe. Three replicates were performed per condition.
Terri Anita Long