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Application: Gene expression microarray analysis
Number of samples: 27
Release date: Jun 2 2009
Last update date: Oct 29 2018
Access: Public
Dataset link Evaluation of methods for amplification of picogram amounts of total RNA for whole genome expression profiling

Experimental Protocol


Four commercial protocols for amplification of picograms amounts of input RNA for microarray expression profiling were evaluated and compared. For each protocol, one RNA amplification was performed from 250 pg, and one from 500 pg of human universal RNA by two operators in two independent laboratories and compared to the amplified aRNA obtained from 2 µg and 100 ng RNA inputs following the standard protocol proposed by Affymetrix. A negative control (amplification without total RNA) and a positive control (if available) were included in each experimental batch. Samples indicating 50, 100, and 1000 pg RNA inputs correspond to 3 additional quantities of total RNA used to synthesise the cDNA target using the nugen protocol for comparison (250, 500 pg + 50, 100, 1000 pg).

Repositories


GEO

GSE15398

ArrayExpress

E-GEOD-15398

BioProject

PRJNA115879

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