|Application:||Gene expression microarray analysis|
|Number of samples:||111|
|Release date:||Feb 4 2010|
|Last update date:||Oct 29 2018|
|Diseases:||Brain Neoplasms, Kidney Failure, Chronic, Neoplasms, Colorectal Neoplasms|
|Dataset link||Stage II and stage III colorectal cancer|
Total of 111 microarray datasets (77 for LCM samples, and 17 pairs for homogenized samples from tumor and adjacent tissues) were normalized using robust multi-array average (RMA) method under R 2.6.2 statistical software together with BioConductor package, as described previously. Then, the gene expression levels were log2-transformed, and 62 control probe sets were removed for further analysis. In order to identify a set of genes associated with development of metastatic recurrence, we performed Wilcoxon rank-sum test for gene expression differences of 54,613 probe sets between recurrence and non-recurrence groups. Similarly, Wilcoxon singed-rank test was conducted to select genes which showed significant expression difference between tumor and adjacent tissue. Then, we selected a set of genes that satisfied both of above two criteria.