Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 64
Release date: Nov 15 2018
Last update date: Nov 30 2018
Access: Public
Genes: BUD13, IRF7
Dataset link Bud13 Promotes a Type I Interferon Response by Countering Intron Retention in Irf7

Experimental Protocol


mRNA profiles of mouse bone-marrow derived macrophages (BMDMs) expressing either knockdown shRNA or control. Stimulations performed with TNFa, Poly(I:C), and CpG. Please note that the -Cyto and -Nuc (in the sample titles) indicates the fraction that was sequenced; '-Cyto' is mRNA from the cytoplasm and '-Nuc' is from the Nucleus. For the TNFa 0 mins - IFNa 120 mins samples, the raw data was simply mapped to generate the splicing data (provided in .tdf files).

Repositories


GEO

GSE122543

ENA

SRP168625

BioProject

PRJNA505512

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Contact


Luke Frankiw