Computational protocol: The locust genome provides insight into swarm formation and long-distance flight

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Protocol publication

[…] We used 106 F1 individuals produced from a cross between two L. migratoria, one from the Hainan province and another from the laboratory-raised population, to perform RADseq. BWA (v. 0.6.2) was used to align the reads to the reference. SAMtools (v. 0.1.18) were used to call single-nucleotide polymorphism and filtering. A custom PERL script was developed to identify segregating polymorphic patterns and classify linkage groups. Marker ordering and spacing were carried out using JoinMap (v. 3.0). […]

Pipeline specifications

Software tools BWA, SAMtools
Application RAD-seq analysis
Organisms Locusta migratoria