Computational protocol: Characterization of Novel Integrons, In1085 and In1086, and the Surrounding Genes in Plasmids from Enterobacteriaceae, and the Role for attCaadA16 Structural Features during attI1 × attC Integration

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[…] Open reading frames (ORFs) were predicted using RAST, and further annotated using BLASTP and BLASTN searches against the UniProtKB/Swiss-Prot, and National Center for Biotechnology Information non-redundant databases. Annotation of drug resistance genes, mobile elements, and other genes was based on CARD analysis, the β-lactamase database, ISfinder, and INTEGRALL. Sequence comparisons were performed using BLASTN and CLUSTALW2. Gene organization diagrams were drawn using Inkscape. […]

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