|Application:||Gene expression microarray analysis|
|Number of samples:||4|
|Release date:||Nov 8 2015|
|Last update date:||Aug 9 2018|
|Chemicals:||Alanine, Amino Acids, Aspartic Acid, Nucleotides|
|Dataset link||Differential gene expression in human THP-1 monocytes expressing the RdRP∆cat mutant transgene compared to THP-1 empty vector control cells|
RdRP cDNAs and a GFP-puromycin fusion protein selection marker were cloned into transfer constructs containing HIV LTRs, a small portion of gag, an RRE, and a cPPT. Transfer constructs were transfected into HEK 293T cells along with packaging construct R∆8.9 and envelope pseudotyping construct expressing VSV-G. RT-normalized amounts of vector were used to transduce THP-1 cells. After selection in puromycin, whole-genome expression profiling was performed on total RNA extracted from the different THP-1 lines.
Meghan M Painter