Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 4
Release date: Nov 8 2015
Last update date: Aug 9 2018
Access: Public
Diseases: Encephalomyelitis
Chemicals: Alanine, Amino Acids, Aspartic Acid, Nucleotides
Dataset link Differential gene expression in human THP-1 monocytes expressing the RdRP∆cat mutant transgene compared to THP-1 empty vector control cells

Experimental Protocol


RdRP cDNAs and a GFP-puromycin fusion protein selection marker were cloned into transfer constructs containing HIV LTRs, a small portion of gag, an RRE, and a cPPT. Transfer constructs were transfected into HEK 293T cells along with packaging construct R∆8.9 and envelope pseudotyping construct expressing VSV-G. RT-normalized amounts of vector were used to transduce THP-1 cells. After selection in puromycin, whole-genome expression profiling was performed on total RNA extracted from the different THP-1 lines.

Repositories


GEO

GSE62754

ArrayExpress

E-GEOD-62754

BioProject

PRJNA265057

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Contact


Meghan Painter
Meghan M Painter