Dataset features

Specifications


Application: RNA-seq analysis, BS-seq analysis
Number of samples: 14
Release date: May 8 2013
Last update date: Oct 10 2018
Access: Public
Chemicals: Cytidine, Cytosine, 5-Methylcytosine
Dataset link AID stabilizes a stem cell phenotype by removing epigenetic memory of secondary pluripotency network genes

Experimental Protocol


Transcriptome sequencing of AID-null tail fibroblasts, wildtype tail fibroblasts, AID-null and wildtype tail fibroblasts reprogrammed for three weeks by ectopic expression of transcription factors Oct4, Sox2, KLf4 and cMyc. Methylation profiling by reduced representation bisulphite seuencing of AID-null tail fibroblasts, wildtype tail fibroblasts, AID-null and wildtype tail fibroblasts reprogrammed for three weeks and AID-null and wildtype clones after three weeks of reprogramming (Picked at two weeks)

Repositories


GEO

GSE46700

ArrayExpress

E-GEOD-46700

ENA

SRP022150

BioProject

PRJNA201855

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Contact


Olivier Elemento

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