Dataset features

Specifications


Application: ChIP-seq analysis
Number of samples: 82
Release date: Aug 22 2015
Last update date: Oct 10 2018
Access: Public
Chemicals: Tyrosine
Dataset link Deregulation of the Ras-Erk Signaling Axis Modulates the Enhancer Landscape [ChIP-seq]

Experimental Protocol


We performed ChIP-seq to assess the global changes in the histone modifications H3K27ac (AC), H3K4me1 (me1), and H3K4me3 (me3) upon loss of feedback regulation through Sprouty (Spry) deletion, and upon unrestrained signaling driven by oncogenic HRasG12V. ChIP-seq was performed in biological duplicate; replicate2 is indicated in the sample name. Spry124fl/fl (VEC) and Spry124-/- (CRE) MEFs were profiled in three conditions: unsynchronized (U), serum starved (S), and serum starved and FGF treated (F). Spry124fl/fl (VEC) MEFs transduced with empty vector (EV) control or HRasG12V (HRas) were profiled in the unsynchronized state.

Repositories


GEO

GSE64190

ArrayExpress

E-GEOD-64190

ENA

SRP051175

BioProject

PRJNA270358

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Contact


Behnam Nabet