Computational protocol: Intravesicular epidermal growth factor receptor subject to retrograde trafficking drives epidermal growth factor-dependent migration

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Protocol publication

[…] Cell culture images were taken at 63X using a Leica SP5-II confocal microscope, courtesy of the Imaging Shared Resource at the Arizona Cancer Center. Tissue images were taken using a Leica DMLB microscope and Leica DFC 310 FX camera mounted on a 1x C-mount using the LAS V4.5 software. Co-association was quantified using JACoP plugin for ImageJ to determine Pearson’s coefficients. […]

Pipeline specifications

Software tools JACoP, ImageJ
Applications Laser scanning microscopy, Microscopic phenotype analysis
Diseases Breast Neoplasms, Neoplasms
Chemicals Tyrosine