|Application:||Gene expression microarray analysis|
|Number of samples:||4|
|Release date:||Sep 1 2012|
|Last update date:||May 4 2018|
|Diseases:||Atherosclerosis, Vascular System Injuries|
|Dataset link||Rapid estrogen receptor signaling is essential for the protective effects of estrogen against vascular injury|
Transgenic disrupting peptide mice (DPM) express the Estrogen Receptor (ER) alpha amino acids 176-253 peptide under CMV promoter control. This peptide blocks interactions between ER and striatin, which inhibits rapid non-genomic signaling by ER to cellular kinases. Female WT and DPM mice (4 mice per sample) were overiectomized, and 1 week later aortas were harvested and treated + 10 nM beta-estradiol (E2) or + EtOH vehicle (Veh) for 4 hrs ex vivo. Total RNA was collected, reverse transcribed to cDNA and used to probe Affymetrix mouse 430.A 2.0 arrays.
Gavin R. Schnitzler