Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 4
Release date: Sep 1 2012
Last update date: May 4 2018
Access: Public
Diseases: Atherosclerosis, Vascular System Injuries
Chemicals: Estrogens
Dataset link Rapid estrogen receptor signaling is essential for the protective effects of estrogen against vascular injury

Experimental Protocol


Transgenic disrupting peptide mice (DPM) express the Estrogen Receptor (ER) alpha amino acids 176-253 peptide under CMV promoter control. This peptide blocks interactions between ER and striatin, which inhibits rapid non-genomic signaling by ER to cellular kinases. Female WT and DPM mice (4 mice per sample) were overiectomized, and 1 week later aortas were harvested and treated + 10 nM beta-estradiol (E2) or + EtOH vehicle (Veh) for 4 hrs ex vivo. Total RNA was collected, reverse transcribed to cDNA and used to probe Affymetrix mouse 430.A 2.0 arrays.

Repositories


GEO

GSE35558

ArrayExpress

E-GEOD-35558

BioProject

PRJNA152677

Download


Contact


Gavin Schnitzler
Gavin R. Schnitzler