Computational protocol: Molecular evolution of candidate male reproductive genes in the brown algal model Ectocarpus

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Protocol publication

[…] Transcriptome data covering male and female gametes [] as well as gametophyte and sporophyte life stages [] were compared to obtain a list of male gamete specific genes. An expression filter of RPKM > 1 was applied to remove background noise. Selected genes were further screened for the presence of transmembrane domains using TMHMM [, ] and signal peptides targeting the outer membrane using HECTAR []. […]

Pipeline specifications

Software tools TMHMM, HECTAR
Applications Protein sequence analysis, Membrane protein analysis
Diseases Disease Models, Animal