|Dataset type:||Genome binding/occupancy profiling by high throughput sequencing|
|Number of samples:||6|
|Release date:||Nov 20 2018|
|Last update date:||Nov 27 2018|
|Diseases:||Neoplasms, Carcinoma, Ductal, Breast|
|Dataset link||Enhanced PAT-ChIP (EPAT-ChIP), a new procedure for reliable genome-wide studies in clinical FFPE tissues|
Our newly designed method EPAT-ChIP was compared with standard PAT-ChIP for validation. Both methods were applied in parallel to the genome-wide study of the low abundant histone mark H3K4me3, known to be associated with active gene promoters, in an archival IBC sample. Then, the comparison between standard PAT-ChIP and EPAT-ChIP was extended to other functionally different histone marks, such as H3K27ac, known to be associated with active promoters and enhancers, and H3K27me3, which characterizes silenced genomic regions.