Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: May 20 2014
Last update date: Aug 9 2018
Access: Public
Diseases: Colonic Diseases, Neoplasms, Colorectal Neoplasms
Chemicals: Zinc
Dataset link Identification of beta-cetenin/TCF4 target genes in LS174T colorectal cancer cells using gene expression arrays

Experimental Protocol


A 4-hydoxy tamoxifen inducible system was used to inducibly block beta-catenin/TCF activity for a period of 36h. Briefly, the ERT2 domain from the pCMV-CRE-ERT2 (Feil et al., 1996) was amplified by PCR and cloned into a modified FUGW lentiviral vector backbone (Lois et al., 2002). NTCF was then amplified byPCR from the pCDNA3.1-NTCF-NLS (van de Wetering et al., 2002) and cloned in frame upstream of the ERT2 from the FUW-CMV-ERT2 (ET) to create the FUW-CMV-NTCF-ERT2 (NET). Total RNA was extracted using the TRIzol® Plus RNA Purification Kit (Life Technologies).

Repositories


GEO

GSE56896

ArrayExpress

E-GEOD-56896

BioProject

PRJNA244875

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Contact


Camille Stephan-Otto Attolini