|Application:||Gene expression microarray analysis|
|Number of samples:||6|
|Release date:||May 20 2014|
|Last update date:||Aug 9 2018|
|Diseases:||Colonic Diseases, Neoplasms, Colorectal Neoplasms|
|Dataset link||Identification of beta-cetenin/TCF4 target genes in LS174T colorectal cancer cells using gene expression arrays|
A 4-hydoxy tamoxifen inducible system was used to inducibly block beta-catenin/TCF activity for a period of 36h. Briefly, the ERT2 domain from the pCMV-CRE-ERT2 (Feil et al., 1996) was amplified by PCR and cloned into a modified FUGW lentiviral vector backbone (Lois et al., 2002). NTCF was then amplified byPCR from the pCDNA3.1-NTCF-NLS (van de Wetering et al., 2002) and cloned in frame upstream of the ERT2 from the FUW-CMV-ERT2 (ET) to create the FUW-CMV-NTCF-ERT2 (NET). Total RNA was extracted using the TRIzol® Plus RNA Purification Kit (Life Technologies).
Camille Stephan-Otto Attolini