Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Aug 21 2007
Last update date: Dec 6 2012
Access: Public
Diseases: Byssinosis, Tick Infestations
Chemicals: Hydrogen Peroxide
Dataset link Rice plants infested with planthoppers

Experimental Protocol


Agilent rice oligo microarray was used to investigate the gene expression profiling in rice plants infested with WBPH or BPH. Total RNA was extracted from pooled leaf blades infested with WBPH or BPH for 24 h and from mock-treated pooled leaf blades. Total RNA (200 ng) was labeled with Cy-3 or Cy-5 using an Agilent low RNA input linear amplification kit. Fluorescently labeled targets were hybridized to Agilent rice oligo microarrays. Hybridization and wash processes were performed according to the manufacturer’s instructions, and hybridized microarrays were scanned using an Agilent DNA microarray scanner. Agilent Feature Extraction software was employed for the image analysis and data extraction processes. Fold changes in expression level in each treatment were compared with those of the respective mock-treated controls. In each treatment, the experiment was performed independently three times.

Repositories


GEO

GSE8811

ArrayExpress

E-GEOD-8811

BioProject

PRJNA102123

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Contact


Masaru Satoh