Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Jan 7 2011
Last update date: Aug 23 2018
Access: Public
Diseases: Airway Obstruction
Dataset link Smooth muscle IL-4 receptor activation induces airway hyper-responsiveness

Experimental Protocol


For the microarray aspect of of the study, there were three groups of mice: 1. IL4R gene knockout (KO) mice 2. WT mice 3. IL4R KO mice that were also transgenic for a gene construct that expressed IL4R under the control of the smooth muscle-specific promoter from the SMP8 gene All mice were subjected to intratracheal IL13 exposure for 7 days, and whole lung RNA was prepared for microarray analysis 24 hours after the last instillation. Per treatment and genotype: Two RNA pools were made from four mice each. These were labeled and hybridized to make a total of 6 microarrays. RNA was labeled with the standard Affymetrix 3' labeling protocol to make cDNA that was hybridized to Mouse MOE 430 plus 2.0 GeneChips. Gene transcripts were identified that differed in their relative expression as a function of IL4R expression on the smooth muscle cells.

Repositories


GEO

GSE26476

ArrayExpress

E-GEOD-26476

BioProject

PRJNA136527

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Contact


Bruce Aronow
Bruce J Aronow

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