|Number of samples:||24|
|Release date:||Jul 10 2018|
|Last update date:||Nov 29 2018|
|Diseases:||Lymphoma, Leukemia-Lymphoma, Adult T-Cell|
|Dataset link||Targeting the HBZ Viral Oncoprotein Transcriptional Network in Adult T-cell leukemia/lymphoma (ChIP-Seq)|
For IRF4 ChIP-seq (n=4), KK1 and ST1 cell line chromatins were immuno-precipitated with normal rabbit IgG (Santa Cruz Biotechnology, sc2027) or IRF4 antibody (Santa Cruz Biotechnology, sc6059 and sc11450 (1:1 mix)). For BATF3 ChIP-seq (n=4), KK1 and ST1 cell line chromatins were immuno-precipitated with normal rabbit IgG (Santa Cruz Biotechnology, sc2027) or BATF3 antibody (R&D, AF7437). For BirA ChIP-seq (n=8), KK1 and ST1 cell lines were engineered to express BirA, transduced with Ctrl, HBZ (pRCMV/TO-flag-BIOTIN-HBZ-puro) or BATF3 (pRCMV/TO-flag-BIOTIN-BATF3-puro) Biotag constructs, and ChIP-seq performed using streptavidin to purify chromatin bound to these isoforms. For H3K27ac and BRD4 ChIP-seq (n=8), KK1 and ST1 cells were pre-treated with either DMSO or JQ1 before being cross-linked and chromatins were later immuno-precipitated with BRD4 antibody (Bethyl, Cat No. A301-985A) or H3K27ac antibody (Abcam, ab4729).