Computational protocol: Genotyping and characterisation of the secretory lipolytic enzymes of Malassezia pachydermatis isolates collected from dogs

Similar protocols

Protocol publication

[…] The isolates’ nucleotide sequences were aligned using the Clustal W program. A phylogenetic tree with 1000 bootstrap replications was constructed using the neighbour-joining method. Genetic distances were calculated using the Kimura two-parameter method, and the resultant tree was drawn with the MEGA (V.5.0) software. […]

Pipeline specifications

Software tools Clustal W, MEGA-V
Applications Phylogenetics, GWAS
Organisms Malassezia pachydermatis, Homo sapiens, Canis lupus familiaris
Diseases Dermatitis, Atopic, Otitis