Computational protocol: Seamless replacement of Autographa californica multiple nucleopolyhedrovirus gp64 with each of five novel type II alphabaculovirus fusion sequences generates pseudotyped virus that fails to transduce mammalian cells

Similar protocols

Protocol publication

[…] Type II alphabaculovirus F and AcMNPV GP64 amino acid sequences were aligned using clustal w (), within MacVector sequence analysis software (MacVector Inc.), and the resulting multiple sequence alignment file was optimized by eye. Using this file as input, phylogenetic analysis (again within MacVector) was undertaken, via neighbour joining with systematic tie-breaking, and the resulting phylogram was rooted with GP64. […]

Pipeline specifications

Software tools Clustal W, MacVector
Application Phylogenetics
Organisms Autographa californica nucleopolyhedrovirus, Homo sapiens