Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 33
Release date: Jan 2 2009
Last update date: Aug 28 2018
Access: Public
Diseases: Deficiency Diseases, Nevus, Intradermal
Dataset link Transcriptional response in skin of mouse 24 hours after intradermal infection with Trypanosoma cruzi

Experimental Protocol


Mice were infected by intradermal injection of 10^6 T. cruzi trypomastigotes in 100uL of saline split between 2 adjacent sites on the shaved side flank. Control mice were injected with an equal volume of saline. 24 hours post-injection approximately 75mm^2 of skin immediately surrounding the injection site was excised and RNA was isolated from the tissue. Balb/c mice were used for most experiments and IFN-gamma KO mice were on the Balb/c background. WT 129 mice were also used as IFNAR-/- mice were on the 129 background. In total 33 arrays were performed. 7 WT (Balb/c) control, 3 Y strain infected, 3 Brazil strain infected, 3 G strain infected, 2 IFN-gamma KO control, 2 IFN-gamma KO infected, 1 NK cell depleted control, 1 NK cell depleted infected, 3 WT (129) control, 3 WT (129) infected, 3 IFNAR KO control, 3 IFNAR KO infected

Repositories


GEO

GSE13522

ArrayExpress

E-GEOD-13522

BioProject

PRJNA110131

Download


Contact


Barbara Burleigh