Dataset features

Specifications


Application: miRNA array analysis
Number of samples: 242
Release date: Jun 1 2015
Last update date: Sep 2 2015
Access: Public
Diseases: Leukemia, Hairy Cell, Leukemia, Lymphocytic, Chronic, B-Cell
Dataset link Association between distinct gene and miRNA expression profiles and utilization of stereotyped subset #4 in the cells of CLL patients [MicroRNA Profiling]

Experimental Protocol


This series of microarray experiments contains the gene expression profiles of purified B-cell chronic lymphocytic leukemia (B-CLL) cells obtained from 217 patients (Binet stage A). Peripheral blood mononuclear cells from CLL patients were isolated by Ficoll-Hypaque (Seromed, Biochrom KG, Berlin, Germany) density-gradient centrifugation. For gene and miRNA expression profiling experiments CLL cells were enriched by negative selection with the EasySep-Human B cell enrichment kit without CD43 depletion (Stem Cell Technologies) using the fully automated protocol of immunomagnetic cell separation with RoboSepTM (Stem Cell Technologies).

Repositories


GEO

GSE51527

BioProject

PRJNA223382

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Contact


Luca Agnelli