|Application:||miRNA array analysis|
|Number of samples:||242|
|Release date:||Jun 1 2015|
|Last update date:||Sep 2 2015|
|Diseases:||Leukemia, Hairy Cell, Leukemia, Lymphocytic, Chronic, B-Cell|
|Dataset link||Association between distinct gene and miRNA expression profiles and utilization of stereotyped subset #4 in the cells of CLL patients [MicroRNA Profiling]|
This series of microarray experiments contains the gene expression profiles of purified B-cell chronic lymphocytic leukemia (B-CLL) cells obtained from 217 patients (Binet stage A). Peripheral blood mononuclear cells from CLL patients were isolated by Ficoll-Hypaque (Seromed, Biochrom KG, Berlin, Germany) density-gradient centrifugation. For gene and miRNA expression profiling experiments CLL cells were enriched by negative selection with the EasySep-Human B cell enrichment kit without CD43 depletion (Stem Cell Technologies) using the fully automated protocol of immunomagnetic cell separation with RoboSepTM (Stem Cell Technologies).