Dataset features


Application: Gene expression microarray analysis
Number of samples: 84
Release date: Apr 10 2017
Last update date: Aug 13 2018
Access: Public
Diseases: Breast Neoplasms, Deficiency Diseases, Neoplasms, Carcinoma, Lobular
Chemicals: Estrogens, Progesterone, Steroids, Mifepristone
Dataset link Posttranslationally modified progesterone receptors direct ligand-specific expression of breast cancer stem cell-associated gene programs

Experimental Protocol

The study contains 3 different cell lines (PR-null, PRB-wildtype, PRB-K388R) tested with 8 different treatments in triplicate (8 x 3 x 3 = 72 individual samples). We also included 12 previously published samples (GSE34148) with the same treatments, which increased our total number of samples to 84. From parental T47D-Y (PR-null) human breast cancer cell lines, we created stable clones expressing either (1) an empty vector (pIRESneo3) or (2) the wild type progesterone receptor isoform B (pIRESneo3-PR-B), or (3) pIRESneo3-PR-B-K388R. These three cell lines were co-treated with either (1) vehicle control (ethanol) or (2) progesterone/R5020 10e-8 M, (3) RU486 10e-7 M, (4) onapristone 10e-7 M, (5) aglepristone 10e-7 M, (6) progesterone plus RU486, (7) progesterone plus onapristone, or (8) progesterone plus aglepristone. Treatments were for 6 hours before total RNA harvest. Standard Illumina HT-12v4 chip was used for gene expression analysis.








Todd Knutson

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