Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 38
Release date: Mar 20 2006
Last update date: Mar 16 2012
Access: Public
Genes: TAF1, KAT2A, TAF1, ELP3, HAT1, HPSE2, KAT5
Taxon: Saccharomyces cerevisiae
Dataset link Genome-wide relationships between TAF1 and histone acetyltransferases in S. cerevisiae

Experimental Protocol


Experiment contains 38 total hybridizations. Yeast cDNA dual channel (Cy3/Cy5) cohybridization. All experiments performed in duplicate (dye swap). Dye swap data was mode normalized using "R" software package and then corrected by B. subtilis spiking controls (added to each culture based on OD600 units). Platform GPL1220: non-commercial nucleotide arrays. PCR amplified yeast (Saccharomyces cerevisiae) genomic DNA chip with ResGen Primer Pairs. Amplification as described at http://cmgm.stanford.edu/pbrown/protocols/2_DNA.html. Printed on aminosaline glass slides by the Penn State University Microarray facility

Repositories


GEO

GSE3345

BioProject

PRJNA93259

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Contact


Melissa Durant