Computational protocol: Poly I: C-activated dendritic cells that were generated in CellGro for use in cancer immunotherapy trials

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Protocol publication

[…] The following monoclonal antibodies (mAbs) against the indicated molecules were used: CD80-FITC, CD83-PE, CD86-PE-Cy5, CD14-PE-Dy590, CD8-APC (Exbio), CD11c-PE, HLA-DR-Alexa700, IFN-γ-PE, CD4-PE_Dy747 (BD Biosciences, San Jose, CA), HLA-A2 flu-MP-PE (Beckman Coulter), and FoxP3-PE (eBioscience). The cells were stained for 30 min at 4°C, washed twice in PBS+0.1% BSA and analyzed using FACSAria (BD Biosciences) with FlowJo software. DCs were gated according to their FSC and SSC properties and as CD11c-positive cells. Appropriate isotype controls and only viable DCs were included in the analysis. [...] Data were analyzed by Man-Whitney non-parametric test using Statistica v.7.1 software. The results were considered statistically significant when p < 0.05. […]

Pipeline specifications

Software tools FlowJo, Statistica
Applications Miscellaneous, Flow cytometry
Diseases Neoplasms, Tay-Sachs Disease, AB Variant
Chemicals Dinoprostone