Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 16
Release date: Dec 30 2010
Last update date: Feb 22 2018
Access: Public
Diseases: Lymphoma, Neoplasms, Lymphoma, Large B-Cell, Diffuse, Lymphoma, B-Cell, Marginal Zone
Chemicals: Amino Acids
Mutations: p|SUB|L|265|P
Dataset link Highly Recurrent MYD88 Mutations That Promote Human Lymphoma Cell Survival

Experimental Protocol


To generate a gene expression signature of MYD88 signaling in ABC DLBCL, the HBL-1 cell line was transduced with retroviral vectors expressing either shMYD88-4 or shMYD88-7. Following puromycin selection, shRNA expression was induced for 24 or 48 hours and gene expression was measured, comparing uninduced (Cy3) to induced (Cy5) cells, using genome-wide Agilent 4x44K oligonucleotide microarrays. A signature of NF-kB signaling in ABC DLBCL was generated by treating HBL-1 cells with the IkB kinase beta inhibitor MLN120B for 2h, 3h, 4h, 6h, 8h, 12h, 16h, and 24h (Cy5), and comparing their gene expression to untreated cells (Cy3). A signature of JAK signaling in ABC DLBCL was generated by treating HBL-1 cells with JAK inhibitor I (5 micromolar; Calbiochem) for 2h, 4h, 6h, and 8h (Cy5) and comparing their gene expression to vehicle-treated cells (DMSO, Cy3). RNA-Seq data not provided.

Repositories


GEO

GSE22900

ArrayExpress

E-GEOD-22900

BioProject

PRJNA127947

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Contact


Louis Staudt
Louis M. Staudt