Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 55
Release date: Aug 17 2012
Last update date: Nov 21 2018
Access: Public
Diseases: Infertility, Myotonic Dystrophy
Chemicals: Nucleotides
Dataset link Transcriptome-wide Regulation of Splicing and mRNA Localization by Muscleblind Proteins

Experimental Protocol


To assess global functions of Muscleblind proteins, RNA-Seq was performed using WT and Mbnl1 KO brain, heart, and muscle (5 mice each). Additionally, C2C12 mouse myoblasts were depleted of Mbnl1, Mbnl2, or both. Subcellular fractionation experiments were performed to analyze mRNA localization following depletion of Mbnl1 and Mbnl2 in C2C12 mouse myoblasts, and following depletion of Mbnl in Drosophila S-2R+ cells. CLIP-Seq was also performed against Mbnl1 in mouse brain, heart, muscle, and C2C12 myoblasts. Finally, ribosome footprinting was performed with C2C12 mouse myoblasts that were depleted of Mbnl1, Mbnl2, or both.

Repositories


GEO

GSE39911

ArrayExpress

E-GEOD-39911

ENA

SRP014709

BioProject

PRJNA172014

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