Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 5
Release date: Nov 1 2017
Last update date: Nov 27 2018
Access: Public
Diseases: Neoplasms by Site
Dataset link A unified activation mechanism for mRNA 3’ end processing and splicing mediated by SR superfamily proteins

Experimental Protocol


Total RNAs from control HEK293T cells, CFIm59 KO cell lines (2 different cell lines), and CFIm68 KO cell lines (2 different cell llines) were fragmented, and reverse transcribed with an oligo(dT) primer containing a linker sequence. cDNAs were circularized, re-linearized by restriction digestion, and PCR amplified to generate libraries. The libraries were sequenced on HiSeq 2000 (single end 150 nucleotides).

Repositories


GEO

GSE101871

ENA

SRP113548

BioProject

PRJNA395735

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