Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 8
Release date: Oct 20 2015
Last update date: Jan 23 2017
Access: Public
Chemicals: Nitrogen, Glutamic Acid
Dataset link The sRNA NsiR4 is involved in nitrogen assimilation control in cyanobacteria by targeting glutamine synthetase inactivating factor IF7

Experimental Protocol


We compared gene expression of Synechocystis sp. PCC 6803 WT with strains showing overexpressed (NsiR4oex) or abolished (∆nsiR4) and re-estbalished (∆nsiR4::oex) NsiR4 expression. For each strain, 2 biological replicates were analyzed. All cultures were supplemented with 2 µM CuSO4 and after 12 hours samples were taken for RNA extraction.

Repositories


GEO

GSE73840

BioProject

PRJNA298210

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Contact


Stephan Klähn
Stephan Klähn