Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 4
Release date: Dec 29 2011
Last update date: Apr 5 2017
Access: Public
Diseases: Melanoma, Neoplasms
Dataset link A novel way for enriching human melanoma precursor cells to identify cellular targets for antineoplastic therapy

Experimental Protocol


To reveal pathways of self-renewal in melanoma we have used two single cell clones that were prepared from primary human melanoma cultures obtained from a primary lesion. These cell clones differed in the self-renewal capacity (in the limiting dilution assay) and in their morphology: high self-renewal/polygonal vs low self-renewal/spindle shaped. We compared the two cell types described above, cultured in either regular RPMI-based media or culture conditions developed to enrich for normal melanocytic precursors (Cook AL, Donatien PD, Smith AG, Murphy M, Jones MK, et al. (2003) Human melanoblasts in culture: expression of BRN2 and synergistic regulation by fibroblast growth factor-2, stem cell factor, and endothelin-3. J Invest Dermatol 121: 1150-1159.), designated MPC (melanoma precursor cells) conditions, for two weeks.

Repositories


GEO

GSE20469

ArrayExpress

E-GEOD-20469

BioProject

PRJNA125015

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Contact


jasmine jacob