Dataset features


Application: Gene expression microarray analysis
Number of samples: 30
Release date: Jan 3 2008
Last update date: Mar 16 2012
Access: Public
Taxon: Portunus pelagicus
Dataset link Differential expression analysis of genes relevant to the moult cycle of Portunus pelagicus

Experimental Protocol

In order to identify differential gene expression across moult stages, two consecutive moult stages were compared on each array in a dual colour (Cy3 and Cy5) experiment. RNA samples were pooled across subjects in order to reduce the effect of biological variation (nine subjects were pooled across a total of three arrays) for each moult stage comparison. Equal amounts of total RNA from three crabs in one moult stage, were pooled, and compared against equal amounts of total RNA pooled from three crabs in another moult stage, on one array. This was repeated three times in total, the different moult stages were labelled with Cy3 or Cy5 respectively. Consecutive moult stages were compared in the following format; postmoult (Cy3) with intermoult (Cy5), intermoult (Cy3) with early premoult (Cy5), early premoult (Cy3) with late premoult (Cy5), late premoult (Cy3) with ecdysis (Cy5), and ecdysis (Cy3) with postmoult (Cy5).








Anna Kuballa

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