Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Apr 21 2011
Last update date: Jul 6 2016
Access: Public
Dataset link Expression data from C. elegans (wild type vs. tag-24)

Experimental Protocol


Gravid adult wild-type and tag-24/octr-1 animals were lysed using a solution of sodium hydroxide and bleach (ratio of 5:2), washed and the eggs were synchronized for 22 hours in S basal liquid medium at room temperature. Synchronized L1 larval animals were placed onto NGM plates seeded with E. coli OP50 and grown at 20°C until the animals had reached the L4 larval stage. Animals were collected and washed with M9 buffer before transferring to NGM plates containing P. aeruginosa PA14 for 4 hours at 25°C. After 4 hours, animals were collected and washed with M9 buffer, and RNA was extracted using TRIzol reagent (Invitrogen). Residual genomic DNA was removed by DNase treatment (Ambion Austin, TX).

Repositories


GEO

GSE27867

ArrayExpress

E-GEOD-27867

BioProject

PRJNA138015

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