Dataset features

Specifications


Application: ChIP-seq analysis
Number of samples: 5
Release date: Aug 29 2018
Last update date: Nov 28 2018
Access: Public
Diseases: Anemia, Iron-Deficiency
Dataset link Genome-wide characterization of the Fur regulatory network reveals a link between catechol degradation and bacillibactin metabolism in Bacillus subtilis

Experimental Protocol


To modulate intracellular iron levels, we employed a high-affinity Fe2+ exporter FrvA from L. monocytogenes to impose iron starvation. Bacillus wild-type cells (with C-terminal FLAG-tagged Fur at its native locus and an IPTG-inducible ectopic copy of frvA integrated at amyE locus) were harvested at 0 and 30 min after IPTG induction to study Fur-dependent regulation under iron sufficient and deficient conditions, respectively.

Repositories


GEO

GSE119163

ENA

SRP159030

BioProject

PRJNA488289

Download


Contact


Hualiang Pi