Computational protocol: HDAC2 and HDAC5 Up-Regulations Modulate Survivin and miR-125a-5p Expressions and Promote Hormone Therapy Resistance in Estrogen Receptor Positive Breast Cancer Cells

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Protocol publication

[…] The overall survival and relapse-free survival of patients with ER+ tamoxifen/endocrine therapy-treated breast cancer stratified by HDAC2, HDAC3, HDAC5, or survivin (BIRC5) expression levels (low and high) were evaluated using Kaplan–Meier analysis from a large publicly available clinical breast cancer microarray online database and web tool (Kaplan Meier plotter) (; ). The overall survival of patients with ER+ tamoxifen-treated breast cancer stratified by miR-125a-5p expression levels (low and high) were evaluated using Kaplan–Meier analysis from a publicly available prognostic miRNA online database and web tool (PROGmiR V2) (). The ROC analysis was constructed to quantify the accuracy of target genes (HDAC2 and BIRC5) using the SigmaPlot SPW10.0 software. The area under the curve (AUC) is a combined measure of sensitivity and specificity between 0 and 1. A test with an AUC value of 1 means perfect accuracy. The Sp1/miR-125a-5p interaction was predicted using miRNA target prediction software (TargetScanHuman 7 and PicTar). […]

Pipeline specifications

Software tools KM plotter, PROGmiR, SigmaPlot, PicTar
Application Miscellaneous
Organisms Homo sapiens
Diseases Breast Neoplasms, Neoplasms
Chemicals Estrogens, Tamoxifen