Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 18
Release date: Mar 24 2010
Last update date: Sep 28 2015
Access: Public
Dataset link Comparative transcriptional profiling of Limbal Epithelial Crypts with rest of the ocular surface epithelial regions.

Experimental Protocol


Samples were prepared from five human ocular surface epithelial regions such as Cornea, Limbus, LEC, LEC Stroma, Conjunctiva. There were four replicates in each groups except Cornea and Conjunctiva with 3 each. The Standard Probe (SP) was prepared from mixing equal amount of Corneal and conjunctival epithelial RNA followed by ethanol precipitation. Samples were labelled with Cy5 dye and Standard Probe with Cy3 Dye. These were mixed in equal amount to prepare hybrid probes which were then hybridised to microarray slide.

Repositories


GEO

GSE19035

ArrayExpress

E-GEOD-19035

BioProject

PRJNA120639

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