Computational protocol: Membrane Phospholipid Fatty Acid Composition Regulates Cardiac SERCA Activity in a Hibernator, the Syrian Hamster (Mesocricetus auratus)

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Protocol publication

[…] Data analyses were carried out using R 2.15.1 . Residuals from statistical models were tested for normality (using Shapiro-Wilk tests), and if necessary, response variables were log-transformed. General linear models with Tukey-like post-hoc multiple comparison tests (R package ‘multcomp’ ) were used to assess differences in SERCA activity and SR PL composition between summer active animals, non-hibernating winter animals, and hibernating animals during IBA, cooling and torpor. For regression analyses we applied ranged major axis (RMA) models (R package ‘lmodel2’ ) to account for measurement errors in both the dependent and predictor variable. Finally, we carried out a path analysis (R package ‘lavaan’ ) to determine the most likely cause and effect relationship between the proportion of a given fatty acid among SR PL, SERCA activity and Tb of the animals. All reported values are means ± standard deviation. […]

Pipeline specifications

Software tools multcomp, lavaan
Application GWAS
Organisms Mesocricetus auratus
Diseases Amino Acid Metabolism, Inborn Errors
Chemicals Fatty Acids, Unsaturated, Linoleic Acid