Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 21
Release date: Feb 17 2015
Last update date: Jul 26 2018
Access: Public
Diseases: Tuberculosis
Dataset link Mycobacterium tuberculosis Chaperonin 60.1 has Bipolar Effects on Human peripheral blood-derived Monocytes

Experimental Protocol


To study the pro-inflammatory activities of Cpn60.1, human monocytes were purified from three healthy donors and incubated with 10 μg/ml M. tuberculosis Cpn60.1 (or 100 ng/ml LPS as a positive control or medium only as a negative control). To study the inhibitory effectds of Cpn60.1 on human monocytes, the cells were pre-exposed to 10μg/ml or 0.1ng/ml of Cpn60.1, washed and incubated with 1ng/ml LPS for further 2 hours. For positive control, the cells were pre-exposed to medium before washing and LPS stimulation while for the negative control, the cells were pre-exposed to medium, washed and again exposed to medium alone.

Repositories


GEO

GSE18794

BioProject

PRJNA121199

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Contact


Ana Cehovin