Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 22
Release date: Mar 26 2009
Last update date: Mar 21 2012
Access: Public
Diseases: Escherichia coli Infections
Chemicals: Amino Acids, Nucleotides
Dataset link Amino acid/Nucleotide perturbation in Escherichia coli

Experimental Protocol


Exponentially growing E. coli cells in M9 minimal medium + 0.4 % glucose were perturbed by the addition of 1 mM amino acid or nucleotide individually, and the transcriptional responses were tracked after 10 minutes. In total, 22 metabolites were tested, including 2-4 biological replicates for each including dye-swaps. In each experiment, the reference channel for the dual channel hybridization were cells at 0 minutes before perturbation.

Repositories


GEO

GSE15409

BioProject

PRJNA117083

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Contact


Dipen Sangurdekar

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