Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 16
Release date: Nov 22 2018
Last update date: Nov 23 2018
Access: Public
Diseases: Breast Neoplasms, Neoplasms
Chemicals: Estradiol, Estrogens
Genes: UNC5A, ESR1, CSF, BCL2, NTN4, ELF5, AKT1
Dataset link Estradiol-regulated Dependence Receptor UNC5A Restricts Estradiol Signaling and Luminal to Basal Transition and Metastasis of Breast Cancer

Experimental Protocol


UNC5A expression was knocked down in MCF-7 and T47D cells by shRNA and stable cells expressing shRNA were generated. The vector expressing cells served as controls (pLKO). Cells were treated with vehicle (ethanol) or estradiol (0.1 nM) for three hours. Total RNA was prepared using RNAeasy kit from Qiagen and subjected to RNA-seq

Repositories


GEO

GSE89700

ENA

SRP093290

BioProject

PRJNA352975

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