Computational protocol: A MYLK variant regulates asthmatic inflammation via alterations in mRNA secondary structure

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Protocol publication

[…] We calculated RNA folding energies (ΔG) using the RNAfold, and mfold program. The recombinant nmMLCK1 constructs (721T or 721C) were transfected into human endothelial cells for mRNA decay and protein expression analysis. In addition, these plasmids were used to transfect the mouse lung in a model of OVA-induced experimental asthma. Asthmatic inflammatory parameters were analyzed as described previously. Detailed methods are in online methods session. […]

Pipeline specifications

Software tools RNAfold, Mfold
Application RNA structure analysis
Organisms Mus musculus, Homo sapiens
Diseases Asthma