Computational protocol: Sexually Dimorphic Expression of eGFP Transgene in the Akr1A1 Locus of Mouse Liver Regulated by Sex Hormone-Related Epigenetic Remodeling

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Protocol publication

[…] The male and female Akr1A1eGFP/+ mice were divided into 3 groups: the intact group and the groups that underwent gonadectomy at 4 and 8 weeks (pre- and post-puberty, respectively). During the operation, the mice were anesthetized by 3% isoflurane, and the testes or ovaries were excised from a 0.5 mm incision. After stitching up the wound, the mice fully recovered within 2 weeks. At 12 weeks of age, the mice were sacrificed, and the EGFP expression in mouse liver was analyzed by IHC and quantified by ImageJ. […]

Pipeline specifications

Software tools Stitching, ImageJ
Application Microscopic phenotype analysis
Organisms Mus musculus
Diseases Liver Diseases
Chemicals Testosterone