Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 16
Release date: Apr 23 2018
Last update date: Nov 28 2018
Access: Public
Diseases: Breast Neoplasms, Neoplasms
Genes: CLK2, TTK, LSM2
Dataset link RNAseq Study in CC-671 Treated Cal-51 Cells

Experimental Protocol


Triple negative breast cancer (TNBC) line CAL51 was grown in DMEM medium containing 10% fetal bovine serum, as recommended by vendor. The growing cells were treated by CC-671 in three biological replicates at the following concentrations and time intervals. The treatment time points were 6 hour and 24 hour. Concentration of compounds used was 3 and 10 uM. Six million cells from each treatment were harvested and RNA was isolated by RNeasy kit. Poly-A selection and strand-specific RNA library construction were performed, followed by multiplexing indexed libraries and sequencing on the HiSeq 2500 with 2x100 bp read lengths. A total of 16 samples were included in this experiment, including 4 treatment groups with three biological replicates and 2 vehicle control groups with two biological replicates

Repositories


GEO

GSE113426

ENA

SRP141379

BioProject

PRJNA451158

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Contact


Tao Shi