Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 14
Release date: Feb 21 2014
Last update date: May 23 2014
Access: Public
Dataset link Evolutionarily conserved C-terminal region of TAF9 is critical for SAGA and TFIID recruitment to promoters and transcriptional activation

Experimental Protocol


This GEO series consists of 14 microarray hybridizations using the Agilent two-color experiment with the Agilent Custom Saccharomyces cerevisiae 8x15k gene expression array. Four biological replicates each for the wild-type (TAF9), the mutant taf9-tCRD2 strain treated or untreated with SM, and the wild-type (TAF9) versus mutant taf9-tCRD2 treated with SM hybridized as dye-swapped replicates. Two biological replicates for wild-type (SPT20) vs spt20D strains treated with SM, and hybridized as dye-swapped replicates to identify the fraction of SAGA dependent genes under amino-acid starvation conditions. The overall aim was to identify genes dependent on the conserved C-terminal region domain of TAF9 and determine their dependence on the SAGA subunit Spt20 for expression.

Repositories


GEO

GSE44544

ArrayExpress

E-GEOD-44544

BioProject

PRJNA190041

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Contact


Krishnamurthy Natarajan
K Natarajan