|Application:||Gene expression microarray analysis|
|Number of samples:||17|
|Release date:||Feb 8 2011|
|Last update date:||Oct 29 2018|
|Diseases:||Breast Neoplasms, Neoplasms|
|Dataset link||Fibroblast triggered gene expression in tumor|
10 tumor samples cocultured with fibroblast were profiled in their gene expression with microarrays, and compared with 7 tumor samples cultured without fibroblast. Immortalized tumor cell lines of varying histologic grade were developed and maintained in a growth median as described in Dairkee, et al., Oncogene, 2007. In the coculture set up, epithelial cells were seeded in 6-well plates, and fibroblasts in 0.4 μm inserts with hanging geometry (BD Biosciences, Franklin Lakes, NJ) at a 3:1 ratio in a common pool of growth medium for 3-day harvests. Controls were comprised of each epithelial sample maintained in the absence of fibroblast-seeded inserts under the same culture conditions.
Citations per year