Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 17
Release date: Feb 8 2011
Last update date: Oct 29 2018
Access: Public
Diseases: Breast Neoplasms, Neoplasms
Dataset link Fibroblast triggered gene expression in tumor

Experimental Protocol


10 tumor samples cocultured with fibroblast were profiled in their gene expression with microarrays, and compared with 7 tumor samples cultured without fibroblast. Immortalized tumor cell lines of varying histologic grade were developed and maintained in a growth median as described in Dairkee, et al., Oncogene, 2007. In the coculture set up, epithelial cells were seeded in 6-well plates, and fibroblasts in 0.4 μm inserts with hanging geometry (BD Biosciences, Franklin Lakes, NJ) at a 3:1 ratio in a common pool of growth medium for 3-day harvests. Controls were comprised of each epithelial sample maintained in the absence of fibroblast-seeded inserts under the same culture conditions.

Repositories


GEO

GSE27018

ArrayExpress

E-GEOD-27018

BioProject

PRJNA137697

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Contact


Junhee Seok

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