Computational protocol: The Latex Protein MLX56 from Mulberry (Morus multicaulis) Protects Plants against Insect Pests and Pathogens

Similar protocols

Protocol publication

[…] RNA was isolated from leaves of M. multicaulis using TRIzol® reagent following the manufacturer’s recommendations (Invitrogen) and digested with DNase I. cDNA was synthesized using 2 μg of total RNA with 100 units of reverse transcriptase M-MLV (Promega) in 20 μL reactions. The specific oligonucleotide primers (MLX56-5′: 5′-GCATGAAGTTTAGAACTCTTCT-3′; MLX56-3′: 5′-TTACATTCGAGCAACTTCCG-3′) were designed based on our available mulberry transcriptome data for PCR amplifications, and the DNA fragments obtained from RT-PCR were subcloned individually into the pMD18-T vector (Invitrogen) resulting in pMD18-MLX56. After transformation, positive clones were selected and further sequenced. The deduced amino acid sequences of MLX56 genes were aligned using DNAMAN multiple alignments program. Structural prediction was performed with SWISS-MODEL tools. […]

Pipeline specifications

Software tools DNAMAN, SWISS-MODEL
Application Protein structure analysis
Organisms Monopterus albus, Arabidopsis thaliana, Botrytis cinerea, Pseudomonas syringae pv. tomato str. DC3000
Diseases Drug-Related Side Effects and Adverse Reactions
Chemicals Amino Acids