Computational protocol: The propensity of the bacterial rodlin protein RdlB to form amyloid fibrils determines its function in Streptomyces coelicolor

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Protocol publication

[…] NMR experiments were performed at 298 K on an Agilent DD2 600 MHz NMR spectrometer. NMR samples contained 400 μM 15N- labeled rodlin protein in 100 mM sodium phosphate buffer pH 7.0 with 5 mM CHAPS, 4 mM DL-Dithiothreitol, 0.5 mM EDTA and 10% (v/v) D2O. All data were processed with NMRPipe and analyzed with NMRViewJ. […]

Pipeline specifications

Software tools NMRPipe, NMRViewJ
Application NMR-based metabolomics
Organisms Bacteria, Streptomyces coelicolor