Computational protocol: Myelination is delayed during postnatal brain development in the mdx mouse model of Duchenne muscular dystrophy

Similar protocols

Protocol publication

[…] Oligodendrocyte α- and β-dystroglycan images were obtained using a zeiss axiocam MRM digital camera and Zeiss Axioplan inverted epifluorescence microscope using 20× and 40× objectives, operating under the control of Axiovision software (Rel. 4.8; Carl Zeiss, Oberkocken, Germany). Oligodendrocyte dystrophin and MBP images were obtained using a Leica SP5 confocal microscope using 20× and 40× objectives, operating under Leica Application Suite AF software. The oligodendrocyte utrophin and all brain section images were obtained using a Leica TCS SP8 X Confocal Microscope using 20×, 40×, and 63× oil immersion objections, operating under Leica Application Suite X (LAS X) software. [...] SigmaPlot11 and Excel were used to perform Student’s two-tailed, paired t-tests and ANOVAs (one-way ANOVA, Student-Newman–Keuls). Compared groups were deemed significantly different if p values had a 0.05 confidence value (* represent p values <0.05, ** represent p values <0.01, and *** represent p values <0.001). The graphs displayed indicate mean values and error bars indicate standard error of the mean (SEM). […]

Pipeline specifications

Software tools LAS X, SigmaPlot
Applications Miscellaneous, Laser scanning microscopy, Microscopic phenotype analysis
Organisms Mus musculus, Homo sapiens
Diseases Brain Diseases, Demyelinating Diseases, Muscular Diseases, Muscular Dystrophy, Duchenne