|Application:||Gene expression microarray analysis|
|Number of samples:||20|
|Release date:||Dec 31 2005|
|Last update date:||Mar 16 2012|
|Genes:||AGRN, LGALS3BP, LGALS9, Set1, BST2|
|Taxon:||Bos taurus, Homo sapiens, Mus musculus|
|Dataset link||Analysis of mRNA expression profiles in bovine endometrium during the pre-attachment period|
Endometrial tissue samples of day 18 pregnant cows (n=4) were compared to non-pregnant controls (n=6). First, subtracted cDNA libraries were produced enriched for cDNAs of genes upregulated either in pregnant animals (18KB) or in the controls (18KO). 1536 clones of every library were picked and PCR-amplified cDNA fragments were spotted on a separate array, respectively. These two arrays were then simultaneously hybridized with P-33 labeled probes derived from the 10 tissue samples (10 hybridizations, 18KB and 18KO array together in 1 hybridization vial). Differentially expressed cDNA clones in comparison of the pregnant and the non-pregnant control group were detected with SAM (Significance analysis of microarrays, Tusher et al. 2001) according to the following parameters: fold-change = 2; FDR = 1%. Signals of these cDNA clones were manually evaluated to eliminate false-positive signal differences. The differentially expressed cDNA clones and some additional interesting cDNA clones were sequenced.