Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Jan 27 2013
Last update date: Jun 8 2015
Access: Public
Diseases: Toxoplasmosis
Chemicals: Cysteine, Tyrosine
Dataset link A nucleolar AAA-NTPase is required for Toxoplasma gondii division

Experimental Protocol


RNA samples were isolated in duplicate from mutant 11-104A4 parasites grown for 32h at permissive (34oC) or non-permissive temperatures (40oC). In addition, a duplicate sample of RNA from mutant 11-104A4 complemented with cosmid TOXOV53 encoding wild type copy of TgNoAP1 similarly grown at 40oC was collected. Samples were hybridized to the Toxoplasma gondii Affymetrix microarray (ToxoGeneChip: http://ancillary.toxodb.org/docs/Array-Tutorial.html). Hybridization data was preprocessed with Robust Multi-array Average (RMA) and normalized using per chip and per gene median polishing and analyzed using the software package GeneSpring GX (Agilent Technologies).

Repositories


GEO

GSE43784

ArrayExpress

E-GEOD-43784

BioProject

PRJNA187427

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Contact


Elena Suvorova
Elena S Suvorova